Process of preparing a substance promoting lactation



Patented Aug. 2, 1938 UNITED, STATES 'VP'ATENT, OFFICE PROCESS OF PREPARING A SUBSTANCE PROMOTING LACTATION Carl Ludwig Lautenschliiger, Frankfort-on-the- Main, and Willy Ludwig, Frankfort-on-the- Main-Hochst, Germany, assignors to Winthrop .Chemical Company, Inc., New York, N. Y., a

corporation of New York No Drawing. Application January 9. 1937, Serial No. 119,868. In Germany January 15, 1936 4 Claims. (01. rev- 74) lating substance from that promoting lactationis especially diflicult.

15 Now we have found that the substance promoting lactation may be prepared by extracting the gonad-stimulating substance from the anterior lobes of the hypophysis and extracting the substance promoting lactation from the thus pretreated gland. As starting material there may, for instance, be used glands which have been prev-treated by the process described in U. S. application Serial No. 94,877, filed August '7, 1936, in the name of Willy Ludwig. According to the 25 process described in that patent application the gonad-stimulating substance and other active substances of the hypophysis are extracted and obtained in a high yield.

During the said process'the anterior lobes oi 30 the hypophysis are extracted at a temperature not exceeding 60 C. with the aid of an organic solvent miscible with water and then with aqueous solvents. If the glands thus pre-treated and freed from the gonad-stimulating substance 35 are treated with a strong alkali within the pH- limits of about 8.0 to about 12.0 in a manner similar to that in which non-pretreated hypphysis has hitherto' been treated, there is obtained an extract of the substance promoting 40 lactation which is to, a large extent free from the gonad-stimulating substance. This extract is further treated, for instance, by precipitating the active substance near the isoelectric point i. e.

feebly alkaline water, suitably with addition of a disinfecting agent, freezing and drying the stituents, that are the gonad-stimulating substance and the substance promoting lactation, 55 without the necessity for a subsequent detriat a pH-value of about 5.5, redissolving it in mental separation of these two constituents from a common solution.

The following examples serve to illustrate the invention, but they are not intended to limit it thereto: 5

1. The moist residue of 15 grams of the anterior lobes of the hypophysis which previously have been extracted in the cold state four times with 15 grams of acetone each and then once with water is stirred with 150 cc. of water and the mixture is adjusted with N-caustic soda solution to a pH-value of 8.5. The mixture is then extracted and centrifuged for one hour at 35 C. This mixture is then extracted twice in the same manner at a pH-value of 9.0 and 10.0

respectively.

The united extracts are again centrifuged, the clear solution is adjusted to a pH-value of 5.0, and the precipitate thus produced is dried with alcohol.

2. The moist residue of 15 grams of the anterior lobes of the hypophysis which previously have been extracted in the cold state four times with 15 grams of acetone each and then once with water is extracted and centrifuged (as described in Example 1) at room temperature and at a pH-value of 8.5 to 11.5. The residue obtained by the subsequent precipitation at a pH-value of 5.0 is redissolved at a pH-value of 8.0 with addition of a disinfecting agent, for instance, sodium 2-ethyl-mercury-mercapto-benzoxazoie-S-carboxylate the solution is brought to a proportion of 1:10000 and dried in a frozen state under reduced pressure.

We claim:

1. Process of preparing a substance promoting lactation which comprises extracting the anterior lobes of the hypophysis at a temperature not exceeding 60 C. with the aid of organic solvents miscible with water, extracting the re- 40 maining gland residue with water and then with an aqueous alkaline solution of a pH-value of about 8'to.about 12 and precipitating the active substance from the alkaline extract by acidification. g

2. Process of preparing a substance promoting -lactation which comprises extracting the anterior lobes of the hypophysis at a temperature not exceeding 60 C. with the aid of organic solvents miscible with water, extracting the remaining gland residue with water and then with an aqueous alkaline solution of a pH-value of about 8 to about 12 and precipitating the active substance from the alkaline extract by acidifying it to a pH-value of 5.5.

i 3. Process of preparing a substance prcmoting iactation: which comprises extracting the anterior lobes of the hypophysis at a temperature not exceeding 60 C. with the aid of organic solvents miscible with water, extracting the remaining gland residue with water and then with an aqueous alkaii solution of a pH-value of about 8 to about 12 and precipitating the active substance from the alkaline extract by acidifying it to a pH-value of 5.5, redissolving the precipi- Itate in an aqueous alkaline solution, freezing and drying the solution. 1*

4. Process of preparing a substance promoting lactation which comprises extracting the anence of a disinfecting agent, freezing and dry- 10 mg the solution.

CARL LUDWiG LAifi'E'NscHLAGEa." WILLY LUDWIG.' 

